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3 edition of Characterization of Bradykinin stimulated responses in cultured human fibroblasts found in the catalog.

Characterization of Bradykinin stimulated responses in cultured human fibroblasts

Beth Gina Etscheid

Characterization of Bradykinin stimulated responses in cultured human fibroblasts

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  • 6 Currently reading

Published .
Written in English


Edition Notes

Statementby Beth Gina Etscheid.
Classifications
LC ClassificationsMicrofilm 90/15 (Q)
The Physical Object
FormatMicroform
Paginationvii, 114 leaves
Number of Pages114
ID Numbers
Open LibraryOL2018818M
LC Control Number90953846

  A Effects of incubation period on the no. of Streptococcus pneumoniae adhering to cultured human tracheal epithelial cells. Fluorescein isothiocyanate (FITC)–labeled S. pneumoniae (10 6 cfu/mL) were added to and coincubated with the cells for periods ranging from 0 to min. B Concentration-response curve of coincubated S. pneumoniae in the adherence of S. pneumoniae to Cited by: Specific, high affinity insulin-like growth factor (IGF) binding proteins are secreted by human fibroblasts in culture. By multiple criteria, the species of IGF binding proteins produced by human fibroblasts are distinct from the HepG2/amniotic fluid IGF binding protein, but share many characteristics with the growth hormone-dependent IGF binding protein forms predominant in normal adult human Cited by: Immunomics is the study of immune system regulation and response to pathogens using genome-wide the rise of genomic and proteomic technologies, scientists have been able to visualize biological networks and infer interrelationships between genes and/or proteins; recently, these technologies have been used to help better understand how the immune system functions and how it .   Fibroblasts cultured from venous ulcers have reduced collagen production response when stimulated with TGF-β and reduced proliferative response with PDFG-BB as compared with controls. Fibroblasts have been isolated from venous stasis ulcers for in vitro assay to evaluate cell cycle protein expression (p21) and modulation by basic fibroblast Cited by:


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Characterization of Bradykinin stimulated responses in cultured human fibroblasts by Beth Gina Etscheid Download PDF EPUB FB2

Bradykinin receptors on cultured human fibroblasts were characterized using [2,3-prolyl-3,H(N)]bradykinin as radioligand. During incubation with intact fibroblasts, intact [3H]bradykinin was lost much more rapidly at 37 degrees than at 4 degrees C as determined by bioassay, high-performance liquid chromatography, and ion-exchange chromatography, and is likely to be by: Transduction of the bradykinin response in human fibroblasts: prolonged elevation of diacylglycerol level and its correlation with protein kinase C by: 8.

Acute responses to bradykinin in human dermal fibroblasts were studied at degrees C using both the patch-clamp technique to monitor ion currents and Fura-2 fluorescence to monitor [Ca2+]i. During subconfluent culture, human dermal fibroblasts can express a diversity of ion channels as described in the preceding paper.

by: Characterization of bradykinin B 2 receptors on human IMR lung fibroblasts: Stimulation of 45 Ca 2+ efflux by D-Phe 7 substituted bradykinin analogues Author links open overlay panel David G. Sawutz a David M. Fauncc a Wayne T.

Houck a Dean Haycock bCited by: Human fetal lung fibroblasts were embedded in type I collagen gels and cultured for 5 days. After release, the floating gels were cultured in the presence of bradykinin.

Bradykinin significantly stimulated contraction in a concentration- and time-dependent manner. Coincubation with Characterization of Bradykinin stimulated responses in cultured human fibroblasts book augmented the effect of 10 −9 and 10 −8 M.

Characterization of the bradykinin receptor involved in inducing TLR4 expression in human gingival fibroblasts. Human gingival fibroblasts (2 × 10 4) were cultivated in 6 wells plates overnight.

Cells were incubated with bradykinin (1 μM); bradykinin + HOE (1 μM) or bradykinin + Lys-[Leu8]des-Arg9-bradykinin (10 μM) for 4 by: 7. This paper describes the pharmacological and functional characterization of bradykinin receptors in human prostate cells.

Human prostate epithelial cells did not express functional bradykinin receptors whereas human prostate stromal cells possessed functional bradykinin B 2 receptors but not bradykinin B 1 by: Human fetal lung fibroblasts were embedded in type I collagen gels and cultured for 5 days.

After release, the floating gels were cultured in the presence of bradykinin. Bradykinin significantly stimulated contraction in a concentration- and time-dependent manner. Coincubation with phosphoramidon augmented the effect of 10 −9 and 10 −8 M Cited by: Abstract.

Bradykinin is a peptide consisting of nine amino acids. It is a member of the kinin family, a class of molecules sometimes considered to be locally acting hormones. Bradykinin acts through cell surface receptors to elicit a series of biological responses, many of which have been well characterized at the whole organ or body by: The results demonstrate bradykinin stimulation of lung fibroblasts to release eotaxin secretion and suggest a role for fibroblasts and eotaxin Characterization of Bradykinin stimulated responses in cultured human fibroblasts book eosinophil recruitment at sites of tissue injury and inflammation.

METHODS Cell cultureCited by: Fibroblasts constitute the majority of non-myocyte cells in the heart, but little is known about the biology of human cardiac fibroblasts. We have cultured and characterized fibroblasts from young adult human heart and compared their biological features with those of cells obtained from the Characterization of Bradykinin stimulated responses in cultured human fibroblasts book tissue of sex- and developmental-stage Cited by: Sawutz DG, Faunce DM, Houck WT, Haycock D.

Characterization of bradykinin B2 receptors on human IMR lung fibroblasts: Stimulation of 45Ca2 Characterization of Bradykinin stimulated responses in cultured human fibroblasts book by D-Phe substituted bradykinin analogues. Eur J Pharmacol Mol Pharmacol ; – Google Scholar. Stimulation of quiescent human fibroblasts with the peptide mitogen bradykinin (BK) led to a biphasic elevation in cellular 1,2-diacylglycerol (DAG), as estimated by either measurement of total.

Novel effects mediated by bradykinin and pharmacological characterization of B2 receptor antagonism in human synovial fibroblasts Article in British Journal of Pharmacology (8) 1. In the present study, we used a microphysiometer to measure bradykinin-induced acidification responses in IMR, a human lung fibroblast cell line, and INT, a human colonic epithelial cell.

Following establishment of a primary culture of fibroblasts, it is necessary to characterize the new strain of cells to ensure their purity and fibroblastic phenotype using immunofluorescence and immunohistochemistry to detect the presence or absence of cell-specific surface by: Characterization of bradykinin receptors showed bradykinin to be extremely potent in exciting the bladder, producing a dose-response curve with an EC(50) of 90 nM, and bradykinin also enhanced.

Bradykinin-induced [Ca 2+ ] i rise in human subcutaneous fibroblasts was concentration-dependently attenuated by the selective B 2 receptor antagonist, HOE (1 and 10 μM, n = 7) (Figure 2B and 2C), whereas selective blockade of the B 1 receptor.

a, BAP1 +/− W-fibroblasts displayed reduced Ca 2+ release from the ER after stimulation with 1 μM bradykinin compared with wild-type fibroblasts (see also Fig.

1e).Cited by: Pharmacological and functional characterization of bradykinin B-2 receptor in human prostate Article in European Journal of Pharmacology (3) November with 29 Reads. Effect of bradykinin (BK) on myofibroblast differentiation.

Time-dependent a) α-smooth muscle actin (α-SMA) protein expression and b) α-SMA + and F-actin + cells and α-SMA and F-actin polymerisation induced by BK in HFL-1 fibroblasts.

Unstimulated cells were used as a Cited by:   Human gingival fibroblasts (HGF) exposed to Pam 2 CSK 4 increased binding sites for bradykinin (BK, B 2 receptor agonist) and des-Arg Lys-bradykinin (DALBK, B 1 receptor agonist).Author: Pedro P.

Souza, Pernilla Lundberg, Inger Lundgren, Fernando A. Magalhäes, Claudio M. Costa-Net. The effect of bacterial toxins on bradykinin-triggered release of arachidonic acid was studied in serum-deprived human foreskin (HSWP) fibroblasts prelabelled with [3H]-arachidonic acid.

An h exposure of HSWP cells to cholera toxin, pertussis toxin, or forskolin enhanced the bradykinin-stimulated release of arachidonic acid and by: Immunopharmacology Comments: Bradykinin is a vasoactive, pain inducing and pro-inflammatory (phlogistic) kinin released during acute inflammation, and its receptors, B 1 and B 2, are expressed on eosinophils, and B 2 receptor antagonist icatibant was evaluated in a Phase 2 trial for osteoarthritis [], but development has not progressed further.

Human BJ fibroblasts were infected for 24 h with recombinant adenoviruses. Infected cells were irradiated with 20 J m -2 ultraviolet light or 15 Gy ionizing radiation, or were cultured Cited by: Schleimer, R.P.

and Rutledge, B.K. Cultured human vascular endothelial cells acquire adhesiveness for neutrophils after stimulation with interleukin I, endotoxin and tumor-promoting phorbol diesters. Immunol. – PubMed Google ScholarAuthor: Paul E. DiCorleto, Carol de la Motte, Ravi Shankar. We have previously characterized the calcium response of cultured human fibroblasts (HSWP cells) to stimulation by the mitogen Lys-bradykinin (BK).Cited by: Both bradykinin receptor genes have been cloned in human and various species and the hydrophobicity prediction for the residues indicates that both bradykinin B1 and B2 receptors have seven helix transmembrane domains, a structure common to other members of the rhodopsin superfamily of G-protein-coupled receptors (G q/11 and G i).

Cell culture. All fibroblast cultures (human neonatal foreskin fibroblasts (ATCC, PCS) and adult dermal fibroblast (ScienCell)) were maintained in fibroblast. Tao et al.

demonstrated increased Ca 2+ responses to bradykinin in ASM cells derived from hyperresponsive inbred rats. Recent studies have shown that cytokines such as TNF-α and IL-1β increase bradykinin receptor expression and function not only in human lung fibroblasts but also in human bronchial ASM cells (48, 53).Cited by:   Lithium inhibits TGF-β-induced gene protein expression in corneal fibroblasts and inhibits TGF-β-induced epithelial mesenchymal transition.

Here, we investigated the effects of LiCl on TGF-β1-mediated signaling pathways and on myofibroblast transdifferentiation of human Tenon’s capsule fibroblasts (HTFs).Cited by: Ensembl ENSG ENSMUSG UniProt P P RefSeq (mRNA) NM_ NM_ RefSeq (protein) NP_ NP_ Location (UCSC) Chr – Mb Chr – Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Bradykinin receptor B 2 is a G-protein coupled receptor for bradykinin, encoded by the BDKRB2 Aliases: BDKRB2, B2R, BK-2, BK2, BKR2.

We have previously observed that gamma-interferon (IFN-γ) inhibited the growth of the intracellular protozoan parasite Toxoplasma gondii in cultured human fibroblasts and that this inhibition was related to the disappearance of tryptophan from the medium with the concomitant appearance of kynurenine and this report, we show that IFN-γ induced an indoleamine 2,3 Cited by: Phospholipid metabolism in bradykinin-stimulated human fibroblasts, I: biphasic formation of diacylglycerol from phosphatidylinositol and phosphatidylcholine, controlled by protein kinase C.

J Biol Chem. ; Google Scholar; 40 Schmitz G, Brüning T, Williamson E, Nowicka G. The role of HDL in reverse cholesterol transport and Cited by:   Additionally, conditioned medium from stimulated fibroblasts significantly increased the proliferation and the formation of the tubular networks of human dermal microvascular endothelial cells.

Further studies by Leu and Leach also found that 45S5 Bioglass/collagen sponges exhibited pro-angiogenic potential both in vitro and in vivo [ 21, 22 ].Cited by: Kinetics of PGE 2 production and COX-2 induction in human PASMC in response to culture with 1 μM BK, 1 ng/ml TGF-β1, or 1 ng/ml IL-1β.

When the cells were cultured with 1 μM BK, 1 ng/ml TGF-β1, or 1 ng/ml IL-1β for 2, 4, 8, 16, and 24 h, there was a significant increase in PGE 2 production compared with time 0 levels and the unstimulated. Bradykinin (BK) has been tobe thought a potent mediator involved in allergic rhinitis because BK was recovered from the nasal lavage fluid of allergic rhinitis patients after allergen provocation and BK receptor antagonists relief nasal allergic symptoms.

Two mammalian BK receptor subtypes, B1 and B2, have been defined based on their pharmacological by:   Intracellular ISG15 is an interferon (IFN)-α/β-inducible ubiquitin-like modifier which can covalently bind other proteins in a process called ISGylation; it is an effector of IFN-α/β-dependent Cited by: BK increases IL-8 production in human lung fibroblasts, cultured human decidua-derived cells, and human airway smooth muscle cells, but not corneal epithelial cells, suggesting that the response to BK is tissue specific.

The effect of BK on IL-8 release has not been studied in airway epithelial by: Human subcutaneous fibroblasts (HSCF) challenged with inflammatory mediators release huge amounts of ATP, which rapidly generates adenosine.

Given the nucleoside’s putative relevance in wound healing, dermal fibrosis, and myofascial pain, we investigated the role of its precursor, AMP, and of its metabolite, inosine, in HSCF cells growth and collagen : Carina Herman-de-Sousa, Ana Rita Pinheiro, Ana Rita Pinheiro, Diogo Paramos-de-Carvalho, Maria Adeli.

Some beneficial effects of angiotensin-I–converting enzyme (ACE, kininase II) pdf therapy are attributed to enhancing the activity of pdf on its B 2 receptor. Independent of inhibition of bradykinin hydrolysis, ACE inhibitors enhance the action of bradykinin on its B 2 receptor by inducing crosstalk between ACE and the receptor.

We investigated whether inhibitors of another Cited by: The Use of Human Foreskins in Medical, Pharmaceutical, and Other Commercial Enterprises. A universal MEDLINE search on 25 April using the key words "Foreskin Fibroblasts" (the term used for harvested foreskins after they are processed for sale) yielded articles.The culture medium was changed to DMEM/% BSA, and the cells were cultured ebook an additional 24 h.

The fibroblasts were stimulated with HAT for 5– min. To examine the effects of the agonists on phosphorylation of MAPK, fibroblasts were stimulated with the agonists in 6-cm by: